Dual-species community formation between F. alocis and A. actinomycetemcomitans analyzed by CLSM.

A. A. actinomycetemcomitans 652 (red, stained with hexidium iodide) was cultured on glass coverslips. F. alocis strains ATCC 35896 (upper left panel) and D-62D (upper right panel) were stained with FITC (green) and reacted with A. actinomycetemcomitans for 24 h, 48 h and 72 h. B. Time-resolved changes in the biovolume of A. actinomycetemcomitans 652, F. alocis ATCC 35896 and D-62D in dual species communities. Data are representative of four independent replicates. P-value compared with control single species communities was calculated by t-test, and significant differences are at p<0.01(**).