Dissociation of the Head3/Neck1 microtubule complex.

The dissociation of the pre-formed Head3/Neck1 microtubule complex was induced by ATP in a stopped-flow apparatus (inset panel A) and followed by the change of the light scattering signal. (A) The graph shows a representative average from 5 traces. The grey curve is a mono-exponential fit to the data that was used to derive k_obs. (B) Graph B shows the hyperbolic dependence of k_obs on the ATP concentration, with a k_max of 0.47 s⁻¹ and a K_1/2 of 105.0 µM ATP.