Figure_7.tif (1.46 MB)
Digestion of human fibronectin by the recombinant human scabies mite aspartic protease N23SsAP.
figure
posted on 2013-11-07, 03:18 authored by Wajahat Mahmood, Linda T. Viberg, Katja Fischer, Shelley F. Walton, Deborah C. HoltRecombinant N23SsAP was tested for its ability to digest human fibronectin. Reactions were run on a 15% non-reducing SDS-PAGE gel and stained with coomassie brilliant blue R-250. Lane 1, molecular weight marker; lane 2, fibrinonectin alone; lanes 3 and 5, fibrinonectin plus N23SsAP; lanes 4 and 6, fibrinonectin plus N23SsAP and pepstatin A, after 4 hours incubation (lanes 3 and 4) and 13 hours incubation (lanes 5 and 6).
