Detection of mineral deposition by mineralizing JPCs (of passage 6).

The upper panel of the figure illustrates the beginning of mineralization at day 12 of osteogenic induction (ob) under both media conditions. Note that precipitate formation originates from only a few cells of the DMEM-cultured monolayer. In contrast, the MC-XF-cultivated monolayer seemed to be purer due to the appearance of mineralization potential originating from almost every cell of the monolayer. 4× magnification. The lower panel of the figure illustrates representative fluorescent stainings of hydroxyapatite formation by OsteoImage in DMEM- and MC-XF-cultured monolayers (growing within coated flasks) at day 20 of osteogenesis. JPCs cultivated under DMEM media conditions showed a stronger mineralization potential. 10× magnification.