Figure_4.tif (1.22 MB)
DNA methylation of EPCAM in whole biopsy samples compared to separated epithelium and negative cell fraction.
figure
posted on 2013-02-08, 00:36 authored by Andreas C. Jenke, Jan Postberg, Timothy Raine, Komal M. Nayak, Malte Molitor, Stefan Wirth, Arthur Kaser, Miles Parkes, Robert B. Heuschkel, Valerie Orth, Matthias ZilbauerA) DNA methylation of 5 CpGs within the EPCAM gene using a pyrosequencing assay. B) Total CpG methylation comparing EPCAM versus NOD1. Assays included 5 CpG sites for EPCAM and 2 for NOD1. C) EPCAM mRNA expression in whole biopsies compared to epithelial positive and negative separated cell fraction. Data is expressed as mean +/− SEM of 5 tissue samples. Differences were considered as statistically significant if p<0.05 (** p<0.001, ***p<0.0001).
