DNAJC14 confers resistance to YFV-induced cell death.

(A) Photographs 7 d after YFV challenge (moi = 1) of SW13 cells transduced with Round 3 of the selected lentiviral cDNA constructs compared to cells transduced with V1-GFP vector control. (B) The cells transduced with the Round 3 lentivirus pool and surviving YFV infection (Rd 3) were expanded and reinfected with YFV at the indicated moi. Crystal violet staining was performed 3 d later. Cells transduced with vector alone serve as a control (V1-GFP). (C) DNA was isolated from naïve SW13 or Round 3 (Rd 3) cells, and the lentiviral insert amplified by PCR. The major band was identified as encoding a truncated hamster DNAJC14. Sizes of the DNA markers (kb) are indicated to the left. (D) A schematic of human DNAJC14 is shown, with the putative transmembrane (TM) domains (gray), J domain (red) with conserved HPD sequence, zinc finger motifs (blue) and Jiv90 domain (orange) indicated. A schematic of the isolated hamster clone, showing homology to amino acids 305 to 702 of human DNAJC14, is shown below.