CzcR is associated to the oprD, phzA1 and czcR promoters.
A) Western blot analysis of the wild-type and the CzcR-HA strain carrying the HA-tagged version integrated into the chromosome. Total proteins from the wild-type or the CzcR-HA strain grown in the presence or absence of 0.5 mM ZnCl2 in LB medium were analyzed by western blot. Antibodies used for the analysis are indicated in the left of the panel. B) Chromosome immunoprecipitation performed on the CzcR-HA strain using anti-HA antibody. Experiment was performed on culture grown in the absence or in the presence of 0.5 mM ZnCl2, as indicated. Results represent the average of 3 independent experiments and standard deviations are indicated.