CzcR is associated to the oprD, phzA1 and czcR promoters.

A) Western blot analysis of the wild-type and the CzcR-HA strain carrying the HA-tagged version integrated into the chromosome. Total proteins from the wild-type or the CzcR-HA strain grown in the presence or absence of 0.5 mM ZnCl₂ in LB medium were analyzed by western blot. Antibodies used for the analysis are indicated in the left of the panel. B) Chromosome immunoprecipitation performed on the CzcR-HA strain using anti-HA antibody. Experiment was performed on culture grown in the absence or in the presence of 0.5 mM ZnCl₂, as indicated. Results represent the average of 3 independent experiments and standard deviations are indicated.