Figure_1.tif (546.05 kB)
Controls used for FISH validation.
figure
posted on 2013-12-04, 02:58 authored by Lysangela R. Alves, Eloise P. Guerra-Slompo, Arthur V. de Oliveira, Juliane S. Malgarin, Samuel Goldenberg, Bruno DallagiovannaA) DNase I treatment before poly-T probe incubation. B) RNase A treatment before poly-T probe incubation. C) Cruzipain sense probes Cy-5 labeled in epimastigotes. D) β-tubulin sense probes Cy-3 labeled in epimastigotes. E) PFR2 sense probes Cy-3 labeled in epimastigotes. F) to J) Merged images, counterstaining with DAPI (blue) was used to identify nuclei (n), kinetoplast (k). Scale bars = 10 µm.
