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Conformational fingerprinting of mutant ACE.

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posted on 21.02.2013 by Sergei M. Danilov, Sergey Kalinin, Zhenlong Chen, Elena I. Vinokour, Andrew B. Nesterovitch, David E. Schwartz, Olivier Gribouval, Marie-Claire Gubler, Richard D. Minshall

Membrane-bound WT and mutant ACE lysates were normalized to achieve 5 mU/ml ACE activity with Z-Phe-His-Leu as substrate and incubated in microtiter plate wells covered with 16 mAbs to human ACE [16], [30] via goat-anti-mouse IgG. Precipitated ACE activity was quantified by fluorimetric plate precipitation assay [30], [54]. Data (mean ± SD of 6–8 independent experiments in duplicate) are expressed as ratio of ACE activity precipitated by mAbs from mutant ACE to that of WT ACE. * p<0.05 vs. WT ACE.