Figure_2.tif (1.29 MB)
Confocal immunolocalization of HA-strep-SLC41A1
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posted on 2013-08-15, 03:59 authored by Martin Kolisek, Gerhard Sponder, Lucia Mastrototaro, Alina Smorodchenko, Pierre Launay, Juergen Vormann, Monika Schweigel-Röntgen(wt) and HA-strep-SLC41A1-p.A350V in +tet (24 h) cells. Strep-tagged wt and p.A350V were immunolabeled with primary mouse anti-strep and secondary GAM Alexa-488 antibodies (green signal). Plasma membranes were fluorescently contrasted with wheat germ agglutinin (WGA) conjugated to Alexa-647 (red signal). Nuclei were stained with DAPI (blue signal). The merged images show that both Alexa-488 and Alexa-647 signals co-localize in +tet cells. Scale bar indicates 10 μm.
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Anatomy and physiologyNeurological systemCentral nervous systemNervous system physiologyPhysiological processeshomeostasisCell physiologygeneticsGenetic mutationGenetics of diseaseMolecular geneticsMolecular cell biologyCell deathMembranes and sortingneuroscienceneurophysiologyneurologyParkinson diseaseimmunolocalization
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