Centromere protein interactions analyzed by F3H assay.

(A) EGFP tagged centromere proteins (bait, green) were recruited to the lac operator repeat array by the GFP-binding protein fused to the Lac repressor (LacI-GBP). Co-expressed mCherry tagged centromere proteins (prey, red) may either interact with the GFP-tagged protein (yellow in the overlay) or may not interact resulting in a disperse distribution. Upper two rows: interaction between EGFP-CENP-O and mCherry-CENP-P, EGFP-CENP-P and mCherry-CENP-O. Lower two rows: EGFP-CENP-Q did not interact with and recruit mCherry-CENP-O to the lac spot, but shows a weak interaction in the reverse combination. For all results see Table 1. Bar: 5 µm. (B): Strong F3H interactions are displayed (++: thick lines, +: thin lines). Black bars: interactions between CENP-PORQU proteins, red arrows: recruitment of CENP-PORQU proteins by CENP-K, -L, and -N. CENP-C is not able to recruit any of the CENP-PORQU proteins (data see Table 2).