Cell differentiation.

A) AdMSCs and FBs were isolated from two patients (P1, P2) and differentiated towards adipocytes, osteoblasts and chondrocytes. RNA was isolated on days 0–7 during differentiation and the resulting 96 RNA samples were used to generate single sequencing library for gene expression analysis. B) In vitro differentiation of AdMSCs (upper panel) and FBs (lower panel) was confirmed by ORO staining of adipocyte, ARS staining of osteoblast and AB staining of chondrocyte cultures on day 14 upon induction of differentiation. The quantified stainings of FBs are represented relative to AdMSCs (lower panel; AdMSC = 1). Abbreviations: P, patient; AdMSC, adipose-derived mesenchymal stem cell; FB, fibroblast; Ad, adipocyte; Os, osteoblast; Ch, chondrocyte; d, day; ORO, Oil Red O; ARS, Alizarin Red S; AB, Alcian Blue.