Breathless regulation of pMoesin does not involve the MAPK pathway signalling.

(A–F″) pMoesin staining in control, btl-RNAi, ras-RNAi, raf-RNAi, mek-RNAi and erk-RNAi expressing terminal cells. pMoesin staining is not seen at the luminal membrane in btl-RNAi (B′). Depletion of Ras (C′), Raf (D′), Mek (E′) and Erk (F′) does not affect pMoesin levels in terminal cells. (G) depletion of Btl, Ras, Raf, Mek or Erk resulted in reduced numbers of terminal branches. An average of 10 terminal cells were analyzed for each genotype. A–F″ are projections of confocal image stacks. Scale bars: (A–F″) 50 µm.