Figure_3.tif (247.13 kB)
Both poly ICLC and CpG-C act as adjuvants for the induction of HPV16-specific binding and neutralizing antibodies.
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posted on 2009-04-10, 00:46 authored by Christiane Stahl-Hennig, Martin Eisenblätter, Edith Jasny, Tamara Rzehak, Klara Tenner-Racz, Christine Trumpfheller, Andres M. Salazar, Klaus Überla, Karen Nieto, Jürgen Kleinschmidt, Reiner Schulte, Lutz Gissmann, Martin Müller, Anna Sacher, Paul Racz, Ralph M. Steinman, Mariagrazia Uguccioni, Ralf IgnatiusHPV16 capsomeres (10 µg/animal) were injected either alone or in combination with 2 mg poly ICLC or 2 mg CpG-C per animal at weeks 0 and 8 (six animals per group), and L1-specific antibodies in plasma samples were measured (A) by ELISA at baseline (pre) and the indicated points in time after the first immunization, and (B) by neutralization assays at week 12. Data given as mean titers +/− SD (A), and as the percentage of neutralization of pseudovirions by serum samples at the dilutions indicated [(B), line, median; *, individual animals)], measured by reduction of SEAP activity (see Materials and Methods) and compared to controls of untreated pseudovirions.
