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Assessment of morpholino efficacy by invitro translation in the presence of [35S]methionine.

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posted on 24.10.2013, 03:39 by Larissa M. Williams, Alicia R. Timme-Laragy, Jared V. Goldstone, Andrew G. McArthur, John J. Stegeman, Roxanna M. Smolowitz, Mark E. Hahn

All morpholinos knock down expression (Nfe2 lanes 2,3; Nrf1a lane 5; Nrf1b lane 7; Nrf3 lane 9) and for nrf1 morpholinos there is specificity for each paralog (data not shown). Scintillation counts, presented as percent decrease over control, give a quantitative measurement of knockdown success.

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