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Analysis of the most representative miRNAs associated to the hStau1 complexes.

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posted on 25.11.2014 by Joan Peredo, Patricia Villacé, Juan Ortín, Susana de Lucas

(A) A bioinformatic analysis was performed using 2 prediction algorithms (TargetScan and DianaLab) and 1 annotation database (Genecodis) to identify target miRNAs sites in the 66 hStau1-associated mRNAs detected in the transcriptomic analysis (see Table S1). The graph represents the number of mRNA with targets for each miRNA. (B) The selected miRNAs were analysed individually by TaqMan RT-qPCR in RNAs isolated from hStau1 complexes purified from HEK293T cells transfected with either pChStaufen1-TAP (white bars), pCTAP plasmid (black bars), or from total cell RNA (grey bars). Values are averages and standard deviations of 3 biological replicates. (C) The relative concentrations of each miRNA in hStau1 versus control TAP complexes are represented as comparison with miR-147a used as an example of miRNA not associated to hStau1. (D) The relative concentrations of each miRNA in RNA samples isolated from purified hStau1 complexes or from total cell RNA are represented.