Figure_5.tif (1.99 MB)
Analysis of signal transduction in muscle lysates from 11, 29 and 54-week female DU6P and Fzt:DU mice.
figure
posted on 2013-02-20, 03:06 authored by Mandy Sawitzky, Anja Zeissler, Martina Langhammer, Maximilian Bielohuby, Peggy Stock, Harald M. Hammon, Solvig Görs, Cornelia C. Metges, Barbara J. M. Stoehr, Martin Bidlingmaier, Carolin Fromm-Dornieden, Bernhard G. Baumgartner, Bruno Christ, Bertram Brenig, Gerhard Binder, Friedrich Metzger, Ulla Renne, Andreas HoeflichWestern blot identified phosphorylated and total expression of the respective signaling molecule. Specific activation was calculated from the ratios of phosphorylated versus total protein. A: GSK3ß (n = 9); B: eIF2α (n = 9); C: p70S6 kinase (n = 3); D: ribosomal protein S6 (n = 6). The inserts provide representative experiments, whereby all samples were studied on the identical membrane. Sample numbers (n) depict the number of samples per age group,the error bars represent SEM (n.c.: not calculated due to low signal intensity).
