Amount and type of transfected SDF.

A) Correction efficiencies after transfecting different amounts of SDF-PCR-WT in D1 cells. Positive events are used to determine the overall modification efficiency respect to D1 control cells transfected with a SDF homologous to mutated eGFP sequence (CTR). B) Different kind of SDFs were tested in D1 cells: double (ds-SDF-PCR-WT 12×10⁶ SDF/cell) or single strand (ss-SDF-PCR-WT 12×10⁶ SDF/cell) PCR fragments and fragment obtained by enzymatic digestion (ds-SDF-DIG-WT 12×10⁶ SDF/cell) were compared to cells transfected with SDF homologous to mutated eGFP sequence (CTR). For representative FACS dot plots see Fig. S3 and S4.