(A) Euclidean distances between transition probability matrices for each feed state. Box plot colors (black, red, blue, orange, grey) are used to help focus trends in specific comparisons groupings, while the line is connecting each box plot mean.

For example, the black boxes are showing that each feed state exhibits an increasing Euclidean distance away from 0-fed, indicating a greater difference in transition probability profiles. The upward trend seen for the black grouping means that as satiety increases, behavior transitions progressively increased in dissimilarity when compared to the 0-fed state. All the colored groupings show similar trends, with ANOVA analysis showing a pValue < 0.05 for all groups. Tukey pairwise comparisons are shown: *p<0.05 and **p<0.01. (B) Penalty cost as a metric of sequence structure similarity. (Bi) Demonstration of PC calculation showing the cost incurred to edit a mismatch and insert/deletion. (Bii) Penalty cost from each feed state was compared to all other feed states. As was the case with (B), the connecting line in (Bii) is connecting each box plot mean. Black, red, and blue groupings show upward trends in the PC, indicating that sequence structure progressively changes as satiety increases (ANOVA analysis showing a pValue < 0.05 for these groups). Tukey pairwise comparisons are shown: *p<0.05 and **p<0.01. No statistical difference was seen in structure when comparing the 0- to 1-fed sequences, nor when comparing the 3- to 4-fed sequences.