99mTc-rCR2 binds to C3d+ RBC.
figureposted on 2013-02-20, 19:25 authored by Adam Badar, Sarah DeFreitas, James M. McDonnell, Norhakim Yahya, David Thakor, Reza Razavi, Richard Smith, Steven Sacks, Gregory E. D. Mullen
Sensitised sheep red blood cells were incubated with human C6 deficient serum to generate C3d deposition on RBC in the absence of cell lysis. 99mTc-rCR2 , 99mTc-C2A or K41E 99mTc-rCR2 were added to 2.5×107 C3d+ cells. The percentage radioactivity bound to C3d+ cells was determined by gamma counting the activity associated with the cell pellet as percentage of total activity added. Error bars represent the standard error of the mean of n = 5.