125I-SI Ang II binding comparison.
Comparison of 125I-SI Ang II binding in the brains of a representative neurolysin KO (right panels) and WT (left panels) mouse strain in the presence of PCMB, losartan, and PD123319. Approximate coordinates relative to Bregma: +3.56 mm for WT and +3.32 mm for KO. Top row shows thionin-stained coronal sections adjacent to the sections used to generate the autoradiograms for “total” (middle panels) and “non-specific” (lower panels) of 125I-SI Ang II binding. Binding is represented in pseudocolor. The vertical calibration bar represents the relationship between 125I-SI Ang II binding density and the color spectrum. The blue horizontal calibration bar shown in the upper left panel = 1 mm. This pattern is repeated for Figures 3–12.