μ-CT analysis and serum markers after ovariectomy.
Sham operation or ovariectomy (OVX) was performed at 5 weeks of age and mice were analyzed at 11 weeks of age. (A-D) μ-CT analyses of femurs in wild-type and Ocn–/–mice with sham operation or OVX. μ-CT images of femoral distal metaphyses (A) and mid-diaphyses (B) are shown. Scale bars = 500 μm. C, Trabecular bone parameters, including the trabecular bone volume (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N). D, Cortical bone parameters including cortical thickness (Ct. Th), the periosteal perimeter (Ps.Pm), and endocortical perimeter (Ec.Pm). wt sham: n = 9, wt OVX: n = 8, Ocn–/–sham: n = 6, Ocn–/–OVX: n = 7. X symbols in box plots show outliers. (TIF)