Yep1 self-interaction, the predicted structure of the Yep1 dimer, and the predicted topology of Yep1.

(A) Yep1 exhibited self-interaction in the BiFC assay. Log-phase cells expressing Yep1-VenusN173 alone, Yep1-VenusC155 alone, or both were examined by fluorescence microscopy. Bar, 5 μm. (B) The predicted aligned error (PAE) plot and pLDDT plot of the AlphaFold-Multimer-predicted structure of the Yep1 homodimer shown in Fig 3F. (C) TOPCONS membrane protein topology prediction for Yep1. (D) Schematics of wild-type and truncated Yep1. (E) Yep1 (35–166) did not interact with full-length Yep1 in a co-immunoprecipitation analysis. (F) Yep1 (79–166) did not interact with full-length Yep1 in a co-immunoprecipitation analysis. (G) Yep1 (1–97) did not exhibit self-interaction in a co-immunoprecipitation analysis. (H) Yep1 (1–113) exhibited self-interaction in a co-immunoprecipitation analysis. Numerical data underlying panels B and C can be found in S1 Data, and raw images for panel E-H can be found in S1 Raw Images.

(TIF)