Yep1 possesses the ability to shape the ER membrane.

(A) Colocalization between Yep1-mCherry and Rtn1-GFP, Yop1-GFP, or Tts1-GFP was quantitated using Pearson’s correlation coefficient (PCC). The PCC values are presented as mean ± SD (n = 10 cells). (B) Quantification of the percentages of cells with extended gaps in images of the midplane in the analysis shown in Fig 3B (more than 300 cells were examined for each sample). (C, D) Quantification of the septum abnormality phenotypes (more than 200 cells with septa were examined for each sample). (E) ER-phagy induced by DTT treatment or nitrogen starvation was largely normal in the absence of Rtn1, Yop1, and Tts1. (F) The ER-phagy defect of yep1Δ cells was not suppressed by the ectopic expression of Rtn1, Yop1, or Tts1. Proteins expressed in yep1Δ were tagged with mCherry, and their expression levels were analyzed by immunoblotting using an antibody against mCherry. Numerical data underlying panels A-D can be found in S1 Data, and raw images for panels E and F can be found in S1 Raw Images.

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