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Yeast and human NTR1 co-localize with nucleolus- and telomere-associated proteins

figure

posted on 2011-12-31, 07:01 authored by Gernot Herrmann, Sanja Kais, Jan Hoffbauer, Kijwasch Shah-Hosseini, Nicole Brüggenolte, Heiko Schober, Margaret Fäsi, Primo Schär

Copyright information:

Taken from "Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism"

Nucleic Acids Research 2007;35(7):2321-2332.

Published online 27 Mar 2007

PMCID:PMC1874655.

() Intracellular localization of Ntr1p and co-localization with other proteins. Upper panel: EGFP-Ntr1p (green) localizes to the nucleus (DAPI, light blue) and forms foci. Middle panel: live cell images of CFP-Nop1p (red, false color) and EGFP-Ntr1p (green). Co-localizing signals are shown in yellow in the merge panel. Lower panel: confocal images of EGFP-Ntr1p (green) in fixed cells immunostained for Rap1p (red). DAPI staining of DNA is shown in blue. Co-localization between the two proteins is shown in yellow on the merge panel. () Intracellular localization of human NTR1 and TRF1. WI26 VA4 cells were co-transfected with expression constructs of ECFP-NTR1 (amino acids 289–580) and RFP-TRF1 (upper three panels). eCFP was used as a control (lower panel). Co-localization in confocal images is shown in yellow on the merge panel and telomeric co-localization is indicated by arrows.