Wound histology

Copyright information:

Taken from "Wound healing and inflammation genes revealed by array analysis of 'macrophageless' null mice"

Genome Biology 2004;6(1):R5-R5.

Published online 23 Dec 2004

PMCID:PMC549066.

Copyright © 2004 Cooper et al.; licensee BioMed Central Ltd.

The location of skin wounds on the back of a neonatal mouse is shown. For the array studies a series of criss-cross wounds were made so that all the skin cells were as close as possible to a wound edge for collection of wound RNA. For hybridization studies and immunohistochemistry we made a series of three incisional wounds, so that transverse sections (broken line) contained the profiles of several wounds. Resin histology through wild-type (left-hand column) and null wounds (right-hand column) at 0.5 h, 3 h, 12 h and 24 h post-wounding. At all stages, arrows mark the epidermal wound edges, which are seen to have met and fused in both genotypes by 24 h. An asterisk (*) marks the migrating epithelial edge. hybridization using a macrophage-specific probe reveals large numbers of macrophages recruited to the granulation tissue in frozen sections through 24 h wounds in wild-type skin (k), while none are present in equivalent tissues of the null mouse (l). Scale bars = (c-j) 400 μM; (k,l) 250 μM.