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Wound healing assay.

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posted on 2017-06-08, 20:09 authored by Rosalba Florio, Laura De Lellis, Viviana di Giacomo, Maria Carmela Di Marcantonio, Loredana Cristiano, Mariangela Basile, Fabio Verginelli, Delfina Verzilli, Alessandra Ammazzalorso, Sampath Chandra Prasad, Amelia Cataldi, Mario Sanna, Annamaria Cimini, Renato Mariani-Costantini, Gabriella Mincione, Alessandro Cama

The effect of 24 μM GW6471 on HNPGL cell migration was evaluated by a monolayer wound-healing assay. Pictures of PTj64i and PTJ86i cells treated with vehicle or with GW6471 were taken at 0, 8 and 16 hours to analyze the dynamics of wound closure (vertical lines indicate wound edges). Histograms represent quantitative analyses of cell migration and are expressed as the ratio of the number of migrated cells in three fields after treatment as compared with vehicle (*p<0.05; ***p<0.001).