Western blot of sheep with clinical signs after inoculation with the WTD scrapie agent.

Western blot comparison of sheep samples inoculated with WTD scrapie to the original No.13-7 classical scrapie inoculum, deer CWD, and x-124 classical scrapie. Monoclonal antibody 12B2 (epitope 93-WGQGG-97) were used for the detection of PrP^Sc. Lane 1, normal sheep brain sample without PK treatment; lanes 2–9 samples of prion-diseased sheep treated with PK; lane 10, normal sheep brain sample treated with PNGase F; lanes 11–17 samples of prion-diseased sheep treated with PK and PNGase F. Sheep numbers 1, 2, 3 and 6 from the present study are indicated in magenta. Sheep inoculated with the WTD scrapie agent have a western blot pattern that is similar to deer CWD. This patterning was not dependent on sheep genotype or the brain region from deer with which they were inoculated. WTDsc, white-tailed deer inoculated with sheep classical scrapie No.13-7. The positions of molecular weight markers 37-, 25-, and 20-kDa are shown to the left of the blots.