ppat.1012031.s006.tif (2.85 MB)
Western blot detection of immune signaling proteins for IECs infected with WT and ece1Δ/Δ.
figure
posted on 2024-03-01, 21:05 authored by Jakob L. Sprague, Tim B. Schille, Stefanie Allert, Verena Trümper, Adrian Lier, Peter Großmann, Emily L. Priest, Antzela Tsavou, Gianni Panagiotou, Julian R. Naglik, Duncan Wilson, Sascha Schäuble, Lydia Kasper, Bernhard Hube(A) Confluent, differentiated C2BBe1 cells were infected with WT (BWP17) and ece1Δ/Δ C. albicans for 6 h and the protein content was sampled. Proteins involved in the damage response of oral epithelial cells were detected with ACT1 serving as a control. n.d. = not determined. (B) Protein levels normalized to actin. For p38, MKP1, EPHA2, EGFR, and AKT the normalized protein level for the phosphorylated protein is presented relative to the total respective protein level.
(TIF)
History
Usage metrics
Categories
Keywords
process remain unclearmicrobial mechanisms behindhuman mucosal surfacesfull virulence potentialfrequently causes infectionsdiv >< pcertain predisposing conditionsopportunistic fungal pathogenpeptide toxin candidalysinintestinal epithelial barrierfungal zinc acquisitionintestinal epithelial cellsmediated barrier protectionrestrict fungal translocationcandida albicans iecs limits candidalysiniecs counteract damagecell damage mediatedintestinal barrieridentified fungalalbicans mediated damagenutrient acquisitioncells servesmediated maintenanceece1 c cell damageusing dualtranslocation acrosstnf signalingspecific responserna sequencingmediates maintenancemain sourceharmless commensalencoding genedisseminated candidiasisdata suggestcell junctions
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC