posted on 2021-03-08, 18:40authored byMichael G. Dorrington, Clinton J. Bradfield, Justin B. Lack, Bin Lin, Jonathan J. Liang, Tregei Starr, Orna Ernst, Julia L. Gross, Jing Sun, Alexandra H. Miller, Olivia Steele-Mortimer, Iain D. C. Fraser
WT, Trif-/-, Sting-/-, and Mavs-/- iBMDMs were infected with J2315 (MOI = 1), fixed at given time points, and stained for LC3b. A) Autophagosome formation was measured by LC3b puncta formation and is presented relative to uninfected cells of each line. B) Representative images of WT, Trif-/-, and Sting-/- iBMDMs at 16 h p.i. showing LC3b (green in merged image) and J2315 (red in merged image) spot formation.