ppat.1008937.s002.tiff (1.29 MB)

Virus was grown on MDCK cells in 6 well plates at either 37°C or 33°C.

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posted on 2021-06-01, 17:41 authored by Daniel H. Goldhill, Ada Yan, Rebecca Frise, Jie Zhou, Jennifer Shelley, Ana Gallego Cortés, Shahjahan Miah, Omolola Akinbami, Monica Galiano, Maria Zambon, Angie Lackenby, Wendy S. Barclay

After 24 hours, the viral supernatant was plaqued and PFU/ml calculated.

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polymerase activity gain wild-type PB 1 segment PA P 653L whilst favipiravir-resistant virus K 229R fitness advantage P 653L PB 1 resistance mutation K 229R pH 1N influenza

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