pone.0262173.g001.tif (771.71 kB)
Viability of 3D human hepatic cultures.
figure
posted on 2022-01-20, 18:26 authored by L. Madison Kirk, Charlotte Mae K. Waits, Alexander C. Bashore, Beverly Dosso, Allison K. Meyers, Antonio C. Renaldo, Thomas J. DePalma, Kelli N. Simms, Nathaniel Hauser, Chia-Chi Chuang Key, Charles E. McCall, John S. Parks, Susan Sergeant, Carl D. Langefeld, Aleksander Skardal, Elaheh RahbarImaging of 3D hepatic constructs was performed using a macro-confocal microscope at 20X magnification. Representative LIVE/DEAD images are shown at days 1, 4, and 7. Live cells are marked as green and dead cells are marked by red nuclei. Large clusters/aggregates of viable hepatocytes are observed. All hepatic constructs remained viable for at least seven days of culture. All scale bars are 100 μm.
History
Usage metrics
Categories
Keywords
specifically linoleic acidgenetic variants nearfatty acid desaturasediv >< pdimensional hepatic constructdimensional culture modelsde novo lipogenesisfatty acid metabolismsignificantly reduced fads1reproducibly displayed allelemodern western dietincluding lipid esterificationdriving human diseasespecific metabolic activityslowest metabolic conversionhuman pufa metabolismlipid metabolismsubsequent metabolismminor alleleincluding obesityhuman pufahuman populationsfads1 activitycardiovascular disease>) displayedvivo tt rs174537 results supportrelevant genepoorly replicatemechanistic explorationinflammatory metabolitesinflammatory mediatorsinflammatory diseasesindividuals homozygoushepatocytes derivedhaplotype blockenriched mediae .,dietary omegadiet interactionscurrent twocholesterol efflux3d hydrogels
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC