Variable GLI1, PTCH1, and reference gene expression in response to Hh pathway stimulation.

GLI1, PTCH1, and reference gene CT (cycle threshold) for ARPE-19, HEK293T, hTERT RPE-1, and NIH/3T3 cell lines with and without stimulation. For cell lines with high CT values (>29), we used a touchdown qPCR method. For each condition (with or without stimulation), we collected mRNA from three batches for each cell line. Normalized GLI1 and PTCH1 expression for each qPCR condition is on the far right. Each symbol in the left panels represents the average CT from three technical replicates for each batch. Each symbol in the right panels represents the expression fold change for each batch.

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