pone.0243333.s002.tif (8.58 MB)
Validation of the BiomarkTM -HD protocol on a cohort of 92 biopsies including 15 positive patients.
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posted on 2021-04-14, 17:55 authored by Julien Fassy, Caroline Lacoux, Sylvie Leroy, Latifa Noussair, Sylvain Hubac, Aurélien Degoutte, Georges Vassaux, Vianney Leclercq, David Rouquié, Charles-Hugo Marquette, Martin Rottman, Patrick Touron, Antoinette Lemoine, Jean-Louis Herrmann, Pascal Barbry, Jean-Louis Nahon, Laure-Emmanuelle Zaragosi, Bernard MariThe correlation of the Cq values obtained for the N primers/probe (BiomarkTM -HD) and the GeneFirst COVID-19 detection kit is presented.
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SARS-CoV -2 variantsoptimized processing bufferassaysetdetectioncapacityinactivating lysis buffersSARS-CoV -2 testing capacitiesRT-qPCRSARS-CoV -2sample PCR reaction volumes4608 qPCR end-pointshigh-throughput SARS-CoV -210 nL-range volumeRNA extraction stepmicrofluidic qPCR testBiomark TM instrumentBiomark TM reactionsresponseprimers
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