pone.0261230.g002.tif (472.64 kB)
Validation of a pooled sample testing strategy for campus-wide SARS-CoV-2 surveillance.
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posted on 2021-12-17, 18:31 authored by Terren Chang, Jolene M. Draper, Anouk Van den Bout, Ellen Kephart, Hannah Maul-Newby, Yvonne Vasquez, Jason Woodbury, Savanna Randi, Martina Pedersen, Maeve Nave, Scott La, Natalie Gallagher, Molly M. McCabe, Namrita Dhillon, Isabel Bjork, Michael Luttrell, Frank Dang, John B. MacMillan, Ralph Green, Elizabeth Miller, Auston M. Kilpatrick, Olena Vaske, Michael D. Stone, Jeremy R. Sanford(A) 10:1 linear pooling strategy. Upto 10 individual sample racks (children) are combined into a single pooled sample (parent) rack. (B) UCSC multiplex N1 assay on 30 individual or pooled positive samples. (C) UCSC multiplex N2 assay on 30 individual or pooled positive samples. (D) Analysis of pooled positive samples with the UCSC multiplex or comparator assay. N1 amplicon (blue) and N2 amplicon (red). (E) Overall performance of the UCSC multiplex assay on thousands of clinical, surveillance and pooled surveillance samples. (F) Turnaround time for both clinical and surveillance samples, rounded to the nearest hour.
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five weeks laterearly april 2020controlling community transmissionautomated sample poolingthousands per day2 diagnostic testing2 surveillancexlink ">testing capacitysystematic screeningsymptomatic individualsscale testingreporting capacityregulatory processesprocessing workflowpowerful toollims ),infectious diseaseexpanded testingcollege campusesbroader communities19 pandemic
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