ppat.1010800.s006.tif (309.73 kB)
VV / B2M colocalization rates.
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posted on 2022-12-27, 18:42 authored by Alejandro Matía, Maria M. Lorenzo, Yolimar C. Romero-Estremera, Juana M. Sánchez-Puig, Angel Zaballos, Rafael BlascoHeLa cells were infected with V-A4-cherry (red) at an m.o.i. of 5 PFU/cell for 1 h at 4°C and unbound virus was removed by washing. Non-permeabilized cells were then incubated for different times at 37°C and stained with anti-B2M to analyze colocalization.
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wide genetic screenstherefore cell survivalscreening top hitsprovide complete resistanceconsistently found βsingle gene knocksignificant gene hitsearly gene expressionfind genes necessaryabsolute infection blockagethus allow cellsdownstream related pathwaysdiv >< pvaccinia virus infectionvaccinia virusb2m geneidentify genesgenes belongingsurvive infectionvirus internalizationvirus entryvirus bindingsurviving cellssignificantly diminishedresults pointrelevant factorreduced probabilityproteins knownpowerful toolsnovel findinghost factorshost factor2 microglobulin&# 946
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