ppat.1009402.g005.tif (639.38 kB)
VP1 I514 is necessary for early morbidity and brain replication in IFN signaling-deficient mice.
figure
posted on 2021-03-11, 18:55 authored by Forrest C. Walker, Ebrahim Hassan, Stefan T. Peterson, Rachel Rodgers, Lawrence A. Schriefer, Cassandra E. Thompson, Yuhao Li, Gowri Kalugotla, Carla Blum-Johnston, Dylan Lawrence, Broc T. McCune, Vincent R. Graziano, Larissa Lushniak, Sanghyun Lee, Alexa N. Roth, Stephanie M. Karst, Timothy J. Nice, Jonathan J. Miner, Craig B. Wilen, Megan T. Baldridge(A) Growth curves of CW3 and CW3I514F in BV2 cells over 72 hpi, inoculated at an MOI of 0.05. Results combined from two independent experiments. (B,C) WT or Ifnar1-/-Ifngr1-/- mice were orally inoculated with 106 PFU of CW3 or CW3I514F, and analyzed for weight loss to 3 dpi (B), and tissue viral levels at 3 dpi (C). N = 7–11 mice per group over two independent experiments with statistical analyses performed as in Fig 3. Dashed lines indicate limit of detection for assays. For panel (B), letters indicate that the data point which they are next to have a statistically significant difference of P < 0.005 in comparison to: a) WT + CW3; b) Ifnar1-/-Ifngr1-/- + CW3I514F. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant.
