ppat.1009784.s004.tif (1.25 MB)
VACV L1R binding to NPC1 and eIF4E as a negative control.
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posted on 2022-01-26, 18:38 authored by Miguel Ángel Cuesta-Geijo, Isabel García-Dorival, Ana del Puerto, Jesús Urquiza, Inmaculada Galindo, Lucía Barrado-Gil, Fátima Lasala, Ana Cayuela, Carlos Oscar S. Sorzano, Carmen Gil, Rafael Delgado, Covadonga Alonso(A) Membranes used to compose S1C Fig (B) Membranes used to compose Fig 3C. Dashed boxes were taken to create the western blot composite figure. Membranes were revealed with mouse anti-Flag antibody, mouse anti-HA antibody and mouse anti-tubulin.
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yet essential processinfectious cycle startsdynamin mediated endocytosisviral nucleic acidsmaller viral factoriesnpc1 ko cellsinternalized via clathrinarrested within endosomeslysosomal membrane proteinsinner viral membranecellular endosomal proteinsasfv proteins e248rmembrane fusion stependosomal proteins mightendosomal membraneendosomal proteinsviral proteinslimiting membranefusion stependosomal exitviral fusionviral coresviral adsorptionasfv proteinsvirion dissolvingupon decapsidationresults suggestph actsouter capsidnpc1 knocknew insightsmultilayer structurelate endosomeincoming virionshost cellfindings paveendocytic pathwayasfv infectivityasf virionalso observed
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