V-ATPase is essential for Yl recycling and yolk granule biogenesis.

Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers with oocyte-specific expression of dsRNA against (A, B) Vha26a or (C-F) Vha68-2 double-labeled for F-Actin (red) and (A, B, E, F) endogenous Rab7 (green) or (C, D) GFP (green) from flies (C) with ectopic expression of 2x-FYVE-GFP or (D) carrying genome-tagging Yl-eGFP-3xHA reporter. (B, C1, D1, F) High-magnification views of the cortex regions highlighted in (A, C, D, E), respectively, as annotated. Genotypes: The samples were from adult females flies heterozygous for both matalpha4-GAL-VP16 driver (BDSC #7062) and the following UAS-transgenic RNAi and PI(3)P or Yl reporter lines: (A, B) P{TRiP.HMS01912}attP2 (#38996). (C) P{w(+mC) = UAS-GFP-myc-2xFYVE}2 (#42172); P{TRiP.HMS01056}attP2 (#34582). (D). P{TRiP.HMS01056}attP2 (#34582);p{mini-W+, yl-eGFP-3xHA}. (E, F) P{TRiP.HMS01056}attP2 (#34582). The sizes of the scale bars as annotated.