pone.0243333.g003.tif (532.07 kB)

Use of the Biomark-based protocol to analyze the host response to SARS-CoV-2 infection at the mRNA and microRNA levels.

figure

posted on 2021-04-14, 17:55 authored by Julien Fassy, Caroline Lacoux, Sylvie Leroy, Latifa Noussair, Sylvain Hubac, Aurélien Degoutte, Georges Vassaux, Vianney Leclercq, David Rouquié, Charles-Hugo Marquette, Martin Rottman, Patrick Touron, Antoinette Lemoine, Jean-Louis Herrmann, Pascal Barbry, Jean-Louis Nahon, Laure-Emmanuelle Zaragosi, Bernard Mari

(A) Overview of the analysis strategy on 72 patient samples. They were divided into 4 groups, according to their viral load, from negative, weak (Cq for viral probes >20), medium (20 > Cq for viral probes >10) and strong (Cq for viral probes < 10) SARS-CoV-2 positive. (B) Typical amplification curves of the different genes (cellular, viral and micro-RNA) on three different SARS-CoV-2 patient status. (C) Modulation of cellular markers in the different groups of patients according to their SARS-CoV-2 viral load. IFIT1 expression was statistically elevated (p < 0.001) in the strong COVID-19-positive samples compared to the three other groups considered separately. The Cq presented are representative of two independent experiments performed in quadruplicate.