Upregulation of BAFF-RFP in cDC and MO myeloid subsets after S. typhimurium infection.

WT and BAFF-RFP^+/- mice were infected i.p. with 500 CFU S. typhimurium and BAFF-RFP⁺ cell populations from naïve, day 3 and day 4 infected spleens were analyzed by flow cytometry. (A) BAFF-RFP expression from cDC subsets (CD8⁺ cDC and CD8^- cDC) and MO subsets (Ly6C^hi MO and Ly6C^hi DC) from naïve and day 4 S. typhimurium infected mice are shown as representative histograms from three independent experiments. Numbers in the plots indicate the percentage of BAFF-RFP⁺ cells. (B) Bar graphs (means ± SEM) indicate the percentage of BAFF-RFP⁺ cells and dotted lines show the percentage of RFP background signal in WT cells. Data are combined from three independent experiments (n = 6–9). Statistics were determined with one-way ANOVA with Holm–Sidak method for multiple comparisons test. * p <0.05, ** p <0.01, *** p <0.001.