Unfolding transition of two independent CPM assays performed in triplicates with 8 μg β-lactoglobulin, which was solubilized in CPM assay dilution buffer.

Mean value of unfolding transition of 8 μg β-lactogolbulin (blue) with standard deviation in black. Melting temperatures were determined by fitting each curve to a Boltzmann sigmoidal equation. The average melting point of these two runs was 72.0°C ± 0.7°C.