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Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy-0

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posted on 2011-12-31, 05:26 authored by Lothar Trieschmann, Alexander Navarrete Santos, Katja Kaschig, Sandra Torkler, Elke Maas, Hermann Schätzl, Gerald Böhm

Copyright information:

Taken from "Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy"

BMC Biotechnology 2005;5():26-26.

Published online 4 Oct 2005

PMCID:PMC1266054.

0 μl PBS (left panel) or in the same volume of serum (right panel), followed by flow cytometry. The measurements are depicted in a Fluorescence 1 (FL1-H) vs. Fluorescence 2 (FL2-H) dot-plot. The number of counts in the area containing specific signals (R2) is given in the figures. Aggregate formation in serum is strongly inhibited.