USUV infection inhibits ArsNa-induced eIF2α phosphorylation in Vero cells.

(A) Vero cells were mock or USUV infected (MOI of 1), treated with ArsNa (0.5 mM), BTdCPU (20 μM), DTT (1 mM), or BEPP (10 μM) for 4 hours at 24 and 48 h p.i., and analyzed by immunofluorescence. Phosphorylated eIF2α was detected with anti-p-eIF2α antibody (green) and USUV-infected cells with anti-dsRNA antibody (red). Nuclei were stained with To-Pro-3 (blue). (B) Quantification of p-eIF2α fluorescence intensity in cells treated as in (A). Scale bars, 25 μm. Statistically significant differences were considered when P<0.05 and marked with an asterisk.(C) Vero cells were mock or USUV infected at an MOI of 1, and treated with ArsNa (0.5 mM) or the with same amount of DMSO for 4 hours at 48 h p.i. Cells were lysed and HRI was detected by western blot using an specific antibody. Membrane was reincubated with an anti-β-actin antibody as a control for protein loading.