posted on 2021-03-08, 18:40authored byMichael G. Dorrington, Clinton J. Bradfield, Justin B. Lack, Bin Lin, Jonathan J. Liang, Tregei Starr, Orna Ernst, Julia L. Gross, Jing Sun, Alexandra H. Miller, Olivia Steele-Mortimer, Iain D. C. Fraser
A,B) WT and Ifnar1-/- iBMDMs were stimulated with IFNγ or regular media for 24 h before being infected with A) dsRed+ J2315 (MOI = 1) or B) GFP+ STm (MOI = 10) for 22 h. Bacterial levels were measured using high-content imaging. C) BMDMs from WT and Ifnar1-/- mice were infected with mCherry WT and ΔSPI2 STm strain SL1344 (MOI = 15) for 24 h in the presence and absence of histidine. Total mCherry signal was measured every 20 min and signal normalized to 1 h pi. * = p≤0.05, ** = p≤0.01, and **** = p≤0.0001 by Mann-Whitney U test.