ppat.1009402.g006.tif (1.1 MB)

Tuft cells are dispensable for, but Stat1 disruption in hematopoietic cells permits, I514- or L514-associated pathogenesis.

figure

posted on 2021-03-11, 18:55 authored by Forrest C. Walker, Ebrahim Hassan, Stefan T. Peterson, Rachel Rodgers, Lawrence A. Schriefer, Cassandra E. Thompson, Yuhao Li, Gowri Kalugotla, Carla Blum-Johnston, Dylan Lawrence, Broc T. McCune, Vincent R. Graziano, Larissa Lushniak, Sanghyun Lee, Alexa N. Roth, Stephanie M. Karst, Timothy J. Nice, Jonathan J. Miner, Craig B. Wilen, Megan T. Baldridge

(A,B)Pou2f3^+/-Stat1^-/- and Pou2f3^-/-Stat1^-/- were orally inoculated with 10⁶ PFU of CR6^F514I and analyzed for survival to 14 dpi (A), splenomegaly (B), stool viral levels (C), and tissue viral levels at 14 dpi (D). N = 11 mice per group over three independent experiments with 5–6 mice per group analyzed at 14 dpi. (E) Pou2f3^+/-Stat1^-/- and Pou2f3^-/-Stat1^-/- were orally inoculated with 10⁶ PFU of WU23 and analyzed for survival to 7 dpi. N = 8–9 mice per group over two independent experiments. (F,G) Stat1^f/f or Stat1^f/f-Vav-iCre mice were orally inoculated with 10⁶ PFU of CR6 or CR6^F514I and analyzed for spleen weights (F) and tissue viral levels at 5 dpi (G). N = 8–10 mice per group over two independent experiments, with statistical analyses performed as in Fig 3. ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant.