Treatment of IECs with another NFκB inhibitor increases host cell damage, loss of barrier integrity, and fungal translocation.

(A) Inhibition of NFκB activation using the NFκB inhibitor SC75741 at concentrations of either 2.5 or 5 μM increased the damage of WT (BWP17) C. albicans, but not for the ece1Δ/Δ strains. LDH release was adjusted by subtracting the release from uninfected and untreated host cells. (B) NFκB inhibition using either concentration also decreased the barrier integrity during infection with both WT and ece1Δ/Δ strains. (C) Fungal translocation was significantly increased during infection with both WT and ece1Δ/Δ C. albicans upon inhibition of NFκB using both concentrations of SC75741. These results match those obtained with the high-affinity NFκB inhibitor quinazoline. All values are shown as the mean with standard deviation. Host-cell damage (A), barrier integrity (B), and fungal translocation (C) data were compared using a one-way ANOVA with a post-hoc Šidák’s multiple comparisons test. Statistical significance: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.

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