ppat.1009430.s001.tif (1.65 MB)
Titration of monocyte:Pf-iRBC ratio for vitro model of monocyte to macrophage differentiation.
figure
posted on 2021-04-06, 17:24 authored by Rajan Guha, Anna Mathioudaki, Safiatou Doumbo, Didier Doumtabe, Jeff Skinner, Gunjan Arora, Shafiuddin Siddiqui, Shanping Li, Kassoum Kayentao, Aissata Ongoiba, Judith Zaugg, Boubacar Traore, Peter D. CromptonElutriated monocytes from healthy U.S. adults (n = 3) were co-cultured with increasing concentrations of Pf-iRBCs. After 24 hours, IL-1β (A), TNF (B) and IL-6 (C) were measured in supernatants, and (D) cell viability was determined by trypan blue dye exclusion and expressed as percent viability.
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