Time-dependent antitrypanosomal in vitro activity for eflornithine and its enantiomers.

Time-dependent in vitro activity for a) racemic eflornithine (blue dashed lines), L-eflornithine (green small dashed lines) and D-eflornithine (red full lines) after 24 h (thick lines), 48 h (medium lines) and 72 h (thin lines) of drug exposure. Parasite growth values are shown as relative fluorescence in the AlamarBlue serial drug dilution assay. Dots represent observed experimental data and lines the model predictions. b) Mean IC₅₀ values with error bars showing the standard error of the estimates for racemic eflornithine (blue dashed line), L-eflornithine (green dotted line) and D-eflornithine (red full line) after different drug exposure times. Please note the log₁₀ scale on the y-axis in S1b Fig.

(TIFF)