Fig 3.tif (1.1 MB)
The sequence of image processing to detect LCRs.
figure
posted on 2017-07-06, 17:29 authored by Alexander V. Maltsev, Sean P. Parsons, Mary S. Kim, Kenta Tsutsui, Michael D. Stern, Edward G. Lakatta, Victor A. Maltsev, Oliver MonfrediThe evolution of a representative LCR profile along Y axes (average Fluo 4 signal within 1 μm band) is shown in blue plots. The LCR signal becomes clearly separated, from the noise and background. Images in panels C,D, and E were contrasted to visualize the detected LCRs.